Review and analysis of metabarcoding primers and DNA reference libraries for mammals occurring in the UK

Tender description

This project aims to: investigate the most frequently used primers when investigating mammals through eDNA metabarcoding; look at the sequence coverage of these markers for mammals occurring in the UK in open access reference libraries and the confidence we can have in the sequences; look at primer bias through in silico analysis; and bring this information together into a single document that can be referred to when providing guidance.   Additional information: To apply for this opportunity you must submit your quotation meeting the requirements detailed in the Request for Quotation (RFQ) attached. Your response must be sent to monitoring.innovation@naturalengland.org.uk by 13:00 on 04/09/2024. If you have any clarification questions linked to this opportunity or the procurement process please submit these via email to monitoring.innovation@naturalengland.org.uk . Please note that, unless commercially sensitive, both the question and the response will be circulated to all tenderers. Contract values stated above are VAT exclusive.