CEFAS24-92 RFQ for marine benthic macroinvertebrate analysis of samples

Tender description

Cefas require a Supplier to process the 36 sediment samples (12 stations with triplicate samples), to extract and identify all biota retained following sieving on a 1.0 mm capture mesh, as per the NMBAQC guidelines detailing extraction and identification Background: The Inner Gabbard East (IGE) dredged material disposal site is located 30 km from Harwich, Essex, UK. The site annually receives large quantities of material from the Harwich approach channel. In June 2024, as part of a Marine Management Organisation (MMO)-funded monitoring program, sediment samples have been collected from within, and from areas surrounding, the site using a 0.1m2 mini Hamon grab. Samples were collected during a survey aboard the RV Cefas Endeavour. In total, 36 samples have been successfully collected (three replicates at 12 stations): the details can be found in table 1 below. The samples have been preserved in formaldehyde shortly after sampling, and all containers have both an internal and external label, clearly indicating the survey and station information. Requirement: Cefas require a Supplier to process the 36 sediment samples (12 stations with triplicate samples), to extract and identify all biota retained following sieving on a 1.0 mm capture mesh, as per the NMBAQC guidelines detailing extraction and identification protocols. In addition to this, there is a requirement to ensure these data are audited as per the NMBAQC Own Samples protocol prior to submission to Cefas (Hall, 2019). http://www.nmbaqcs.org/media/1764/os686970_summary-report.pdf. Enumeration, identification and biomassing to the lowest possible taxonomic level should be carried out following the NMBAQC Processing Requirement Protocol and Taxonomic Discrimination Protocol. Biomass (wet weight in grams to at least 4 d.p.) is required for all enumerable taxa. Balances must be serviced annually and a valid service certificate must be supplied. A single reference collection of all species/taxa identified is required to be returned to the Cefas laboratories in Lowestoft in an appropriate preservation liquid (i.e. 100% IDA or 70% IMS, 20% water and 10% glycerol). The reference collection should contain, as a minimum, the species name, name of identifier/ supervisor, station code, cruise code, date of collection and name of species identification verifier. A list of all taxa in the reference collection is also requested, in electronic form, with these details included. Notable taxa should be highlighted here such as non-native species and new or unusual records. Non-native species should be added to the Non-native Species Information Portal (NNSIP) (hosted on the Non-native Species Secretariat website, http://www.nonnativespecies.org/index.cfm?sectionid=81). The abundance and biomass data matrices should be in completed with, at minimum, the detail in the exemplar provided and then sent via email following completion (full details to be provided at kick off meeting). All species names must be run through the WoRMS ‘Match Taxa’ tool so that all naming is current (http://www.marinespecies.org/aphia.php?p=match). Samples are available for collection, by the successful Supplier, as soon as possible following Contract Award, from the storage facility in Lowestoft. Please include a cost to acknowledge this requirement within the RFQ Part 2 (Pricing). Samples must be transported safely and securely. Full details of the sample collection are to be confirmed at kick off meeting Download the Bidder Pack at https://defra-family.force.com/s/Welcome and search for CEFAS24-92 under opportunities.