Multi-point confocal Microscope

Tender description

The University is looking to purchase a DragonFly505 multi-point confocal microscope. The system has a multi-modal 2-camera confocal microscope w/ laser Widefield, TIRF, and super-resolution capability. With motorized 1, 1.5 and 2x optical camera zoom to match Nyquist sampling and motorized illumination zoom for high power density illumination of dSTORM samples. Lot 1: The University is looking to purchase a DragonFly505 multi-point confocal microscope. The system has a multi-modal 2-camera confocal microscope w/ laser Widefield, TIRF, and super-resolution capability. With motorized 1, 1.5 and 2x optical camera zoom to match Nyquist sampling and motorized illumination zoom for high power density illumination of dSTORM samples. The equipment comes under our budget of £300,000 and includes the following required features to enable our research outputs using one system: • 3D localisation astigmatism optics for 3D dSTORM • Spinning disk confocal, microlens enhanced to maximise excitation efficiency • 25um and 40um pinhole click-switchable options for high and low magnification objectives. • Borealis flat-field illumination for seamless image tiling, without the need for shading correction • NIR extended range, >730nm laser excitation possible that is compatible with the flat-field illumination solution, no requirement for second laser input • Dual channel simultaneous TIRF via the microscope imaging left side port, no microscope epi-turret dichroic required to ensure TIRF field flatness • Vacuum sealed 1kx1k 13um pixel EMCCD camera and vacuum sealed 2kx2k 6.5um back illuminated sCMOS camera • Super resolution SRRF Stream+ on board both cameras , with instant feedback on the output image in the acquisition software • Provides 3D visualisation, as 3D data is being acquired in real time • Real time GPU accelerated deconvolution with parallel processing during image capture • Output image data is optimised for use in IMARIS analysis software