Laboratory, optical and precision equipments (excl. glasses)

Tender description

Benchtop high throughput sequencing machine to determine the changes in the profile of mRNAs expression in cell and tissue samples exposed to oligonucleotide-based therapeutics (and relevant controls). In contrast to genomic DNA sequencing which requires long-reads and a low-throughput, sequencing of mRNA requires large numbers (high-throughput) of sequencing to undertake accurate quantification (>400M reads per run). Includes 4 years post warranty service cover. Purchase of a benchtop DNA short-read, high-throughput sequencer. The primary purpose of this sequencing machine is to determine the changes in the profile of mRNAs expression in cell and tissue samples exposed to oligonucleotide-based therapeutics (and relevant controls). In contrast to genomic DNA sequencing which requires long-reads and a low-throughput, sequencing of mRNA requires large numbers (high-throughput) of sequencing, it order to undertake accurate quantification. We therefore require an easy to run/maintain high-throughput short-read sequencing machine (> 400 million reads per run). In addition, this machine should be able to undertake paired-end sequencing (which improves alignment accuracy), of varying lengths (which permits multiple applications and cost savings) and of high quality (> 80 % of reads at Q > 30). We also require comprehensive and automated bioinformatics support that converts raw sequencing data (BCL files) into FASTQ files, that are then aligned to relevant genomes (to produce BAM files) before quantification of changes in the mRNA expression profile. Must include 4 years post warranty service cover.