GB-Glasgow: Supply of Flow Cytometers

Tender description

Scottish Water Scientific Services is responsible for collecting and analysing water and wastewater samples for regulatory compliance monitoring and operational purposes. The laboratory is accredited to ISO17025 and ISO14001. The Microbiology laboratory currently follows a method describing the detection and assessment of the total (LIVE and DEAD) and intact (LIVE) bacteria cell numbers of potable water samples using a flow cytometer. The flow cytometer applies a laser beam to the sample and detects fluorescent signals emitted from stained cells. Two DNA intercalating dyes are used to stain the cells depending on their condition and distinguish between intact and compromised cells. As part of Scottish Water's flow cytometry upgrade project, we have been asked to evaluate our flow cytometry capabilities to ensure public health protection. It is important that our laboratory continues to focus on shortening analysis times whilst retaining limits of detection/recoveries. Scottish Water are looking into purchasing three Flow cytometers capable of rapid detection of total and intact bacteria cell numbers in potable water providing minimal variation whilst minimising sample loss to provide increased productivity. The technical assessment will be made on the information provided during laboratory demonstrations and the responses to this tender specification. The evidence provided should include a summary of the data produced in the laboratory demonstration (highlighting reproducibility, precision, instrument sensitivity and accuracy, robustness and potential time saving. The responses to the questions in this tender document should be comprehensive and should include all details of any software provided, and training provided, support for the method implementation, and instrument specification.