Research services

Tender description

There is a requirement to provide DNA sequencing, Oligonucleotides synthesis and custom DNA/RNA synthesis for laboratories undertaking research in the UK Public Sector. This broad and diverse requirement extends from contracted Government and academic research laboratories involved with the life sciences, biological, medical and environmental research, through to chemical and physical research. UK SBS wish to put in place a framework for the purchase a range of user selectable DNA Sequencing services (Sanger/ ~800-1000 base pair read) from a range of samples and formats including tubes, microtiter plates, preparation & extractions services including from colonies and tissue, with the capability of sequencing from High GC and AT samples. UK SBS wish to put in place a framework for the purchase a range of user selectable (via supplier engagement) range of Custom Gene Synthesis. Custom Gene fragment synthesis (sub 2000 base pairs) For the above sub-lot, a range of user selectable additional services should be available (but are not mandatory). This could include but is not limited to the following: • Genes produced with additional synthetic Nucleotides (AGTC + XY) • Custom vector production • Codon optimized sequences – all species • Site-directed mutagenesis • Bacterial, yeast, Baculovirus and mammalian gene expression services • Complex sequence synthesis - GC rich, AT rich, complex repeats & hairpin structures and toxic sequences UK SBS wish to put in place a framework for the purchase a range of user selectable (via supplier engagement) range of Custom Gene Synthesis. Custom Gene synthesis (2001 - 3000 base pairs) For the above sub-lot, a range of user selectable additional services should be available (but are not mandatory). This could include but is not limited to the following: • Genes produced with additional synthetic Nucleotides (AGTC + XY) • Custom vector production • Codon optimized sequences – all species • Site-directed mutagenesis • Bacterial, yeast, Baculovirus and mammalian gene expression services • Complex sequence synthesis - GC rich, AT rich, complex repeats & hairpin structures and toxic sequences UK SBS wish to put in place a framework for the purchase a range of user selectable (via supplier engagement) range of Custom Gene Synthesis. Custom Gene synthesis (3001-5000 base pairs) For the above sub-lot, a range of user selectable additional services should be available (but are not mandatory). This could include but is not limited to the following: • Genes produced with additional synthetic Nucleotides (AGTC + XY) • Custom vector production • Codon optimized sequences – all species • Site-directed mutagenesis • Bacterial, yeast, Baculovirus and mammalian gene expression services • Complex sequence synthesis - GC rich, AT rich, complex repeats & hairpin structures and toxic sequences UK SBS wish to put in place a framework for the purchase a range of user selectable (via supplier engagement) range of Custom Gene Synthesis. Custom Gene synthesis (5001+ bp) For the above sub-lot, a range of user selectable additional services should be available (but are not mandatory). This could include but is not limited to the following: • Genes produced with additional synthetic Nucleotides (AGTC + XY) • Custom vector production • Codon optimized sequences – all species • Site-directed mutagenesis • Bacterial, yeast, Baculovirus and mammalian gene expression services • Complex sequence synthesis - GC rich, AT rich, complex repeats & hairpin structures and toxic sequences UK SBS wish to put in place a framework for the purchase a range of Gene Specific DNA Methylation services are required to include, But not limited to: O Perform Bisulfite modification O Perform PCR reactions O Perform Pyrosequencing analysis UK SBS wish to put in place a framework for the purchase Global DNA Methylation A range of global DNA methylation services are required to include, but not limited to: O LC MS DNA-methylation analysis O ELISA that enables the direct quantitation of genomic DNA methylation. O PCR based methods e.g. Alu and LINE1 O Methylation sensitive restriction assays eg luminometric methylation assay (LUMA). UK SBS wish to put in place a framework for the purchase Allele quantification A range of Allele quantification services are required to include, but not limited to: O SNP detection and quantification O Assay development for SNP detection and quantification from pooled DNA with a defined 100bp target region in murine DNA O Validation of the above assay O Sample testing if required for the above assay. UK SBS wish to put in place a framework for the purchase of a range of custom Oligonucleotide synthesis services including Custom DNA oligo’s in tubes, plates and RNA/siRNA, to be user selectable via supplier engagement with a range of purification and modification options. Synthesis of DNA oligonucleotides. The production of custom user defined oligonucleotides in the following ranges are required (Inc. plates) • 0.01UM bases standard • 0.05UM bases standard • 0.2UM bases standard • 1.0UM bases standard • 0.025 UM / 3OD • Microtiter plates UK SBS wish to put in place a framework for the purchase of a range of custom Oligonucleotide synthesis services including Custom DNA oligo’s in tubes, plates and RNA/siRNA, to be user selectable via supplier engagement with a range of purification and modification options. Synthesis of Custom RNA oligo’s (both Single and double stranded RNA oligoes, siRNA) the supply of siRNA Libraries and the production of custom SiRNA Libraries The production of custom user defined oligonucleotides in the following ranges are required (Inc. plates) • 0.01UM bases standard • 0.05UM bases standard • 0.2UM bases standard • 1.0UM bases standard • 0.025 UM / 3OD • Microtiter plates