Laboratory reagents

Tender description

NWSSP-PS is issuing this PIN as a Request for Information (RFI) on behalf of the All Wales Medical Genetics Service (AWMGS) to engage with the market to seek information and to gain a better understanding of the capacity in delivering Whole Exome and Whole Genome library preparation kit for use by the laboratory. Please note: the PIN is not a call for competition; you will need to express your interest in any future tender once the Contract Notice is published in OJEU and on Sell2Wales. The PIN/RFI invites individuals and organisations (I and O) to express an interest in this RFI exercise only. It is not part of any pre-qualification/selection process. An expression of interest is no indication of a commitment to participate in the RFI process nor does it infer any preferential or special status on those suppliers who express an interest in the RFI exercise. This PIN/RFI is intended as an awareness, communication and information gathering exercise. The laboratory intends to procure a whole exome and whole genome library preparation kit. These solutions will demonstrate acceptable data QC metrics with DNA extracted predominantly from whole blood but also include amniotic fluid and CVS samples, FFPE and saliva samples. In view of the samples to be used the input yield of DNA required must be as low as possible. The library preparation process should be compatible with automation using existing laboratory robotic equipment, Hamilton STAR. The AWMGS’ procurement process may include a technical questionnaire along with the processing of samples in house followed by assessment of data quality. The AWMGS wish to invite interested suppliers to present their solution to the laboratory to enable information gathering to ensure the market is capable of meeting the needs of the laboratory. Information of particular interest: 1) Percentage of the exome/genome is covered at least 30x; 2) Percentage of Panel App genes with strong evidence of disease association (“green genes”) are covered at at least 30x; 3) The sequencing criteria that would generate the above (Q1 and Q2) coverage; 4) The sensitivity and specificity of variant calling against a stated reference genome for: i. SNVs; ii. Indels.