Laboratory, optical and precision equipments (excl. glasses)

Tender description

The Astbury BioStructure Laboratory (ABSL) at the University of Leeds has identified a requirement for 4 instruments to perform correlative light and electron microscopy (CLEM) and electron microscopy of cells and tissues. These instruments are: — A high-pressure freezer to allow rapid freezing of large and/or labile specimens whilst minimizing structural deformation; — And a freeze-substitution unit for low-temperature substitution of water by dehydrating agents and fixatives; — A cryo-ultramicrotome, for room-temperature and cryo-sectioning of samples for (cryo-)EM imaging; — And a cryo-CLEM system, to image cryo-fixed samples by fluorescent microscopy, identifying interesting biological events for further analysis at high-resolution by cryo-EM. — 1 high-pressure freezer to allow rapid freezing of large and/or labile specimens whilst minimizing structural deformation; — 1 freeze-substitution unit for low-temperature substitution of water by dehydrating agents and fixatives; — One cryo-ultramicrotome, for room-temperature and cryo-sectioning of samples for (cryo-)EM imaging; — One cryo-CLEM system, to image cryo-fixed samples by fluorescent microscopy, identifying interesting biological events for further analysis at high-resolution by cryo-EM.